If you're sitting AQA Combined Science: Trilogy (spec code 8464), there are 21 required practicals you need to know. You don't carry any of them out in the exam. Instead, questions about method, variables, results and conclusions turn up across all six papers, and they can appear anywhere a related topic shows up.
AQA numbers the practicals 1 to 21 in one continuous run. Biology takes 1 to 7, Chemistry takes 8 to 13, Physics takes 14 to 21. The rest of this guide walks through all 21 in that order, flags which paper each sits under, and reconciles the ones that get dropped if you're on Trilogy rather than Triple.
If you're on a different board, we've got separate guides for OCR Gateway (spec A) and OCR Twenty First Century (spec B). For Edexcel 1SC0, use our Edexcel Combined Science core practicals guide instead. The language also shifts between boards: AQA says "required practicals", Edexcel says "core practicals", OCR says "PAGs" (Practical Activity Groups). Same idea, different names.
This article was last checked against the AQA Combined Science: Trilogy (8464) specification on 14 October 2026. Specifications and assessment detail change often, so cross-check the current AQA specification page before relying on any specific detail.
Trilogy is the double-award version of AQA GCSE Science. You come out with two grades (from 9-9 down to 1-1) on one certificate. On the Triple route, you sit three separate GCSEs with 28 required practicals in total: 10 Biology, 8 Chemistry, 10 Physics. The reconciliation sits further down.
All 21 AQA Trilogy required practicals
The table below lists every required practical in AQA's own 1 to 21 numbering. Each one belongs to a topic area, and questions about it tend to appear in the section of the paper that covers that topic. Each of the six papers is 1 hour 15 minutes long, worth 70 marks, and accounts for 16.7% of the overall grade.
| No. | Required practical | Science | Paper |
|---|---|---|---|
| 1 | Microscopy: observe, draw and label plant or animal cells with a scale | Biology | 1 |
| 2 | Osmosis: effect of sugar or salt solutions on plant tissue mass | Biology | 1 |
| 3 | Food tests: Benedict's, iodine, Biuret and the ethanol emulsion test | Biology | 1 |
| 4 | Enzymes: effect of pH on amylase activity | Biology | 1 |
| 5 | Photosynthesis: effect of light intensity on the rate of photosynthesis | Biology | 1 |
| 6 | Reaction time: effect of a factor on human reaction time | Biology | 2 |
| 7 | Field investigations: measuring population size using sampling | Biology | 2 |
| 8 | Making salts: pure, dry sample of a soluble salt from an insoluble oxide or carbonate | Chemistry | 1 |
| 9 | Electrolysis of aqueous solutions using inert electrodes | Chemistry | 1 |
| 10 | Temperature changes: variables affecting energy change in reacting solutions | Chemistry | 1 |
| 11 | Rates of reaction: effect of concentration on rate (gas volume or turbidity) | Chemistry | 2 |
| 12 | Chromatography: separating mixtures and calculating R<sub>f</sub> values | Chemistry | 2 |
| 13 | Water analysis: pH, dissolved solids and distillation of water samples | Chemistry | 2 |
| 14 | Specific heat capacity of one or more materials | Physics | 1 |
| 15 | Resistance: factors affecting the resistance of electrical circuits | Physics | 1 |
| 16 | I–V characteristics: filament lamp, diode and a resistor at constant temperature | Physics | 1 |
| 17 | Density: densities of regular and irregular solids and of liquids | Physics | 1 |
| 18 | Force and extension of a spring | Physics | 2 |
| 19 | Acceleration: force vs acceleration and mass vs acceleration | Physics | 2 |
| 20 | Waves: frequency, wavelength and speed in a ripple tank and in a solid | Physics | 2 |
| 21 | Infrared: absorption and emission by different surfaces | Physics | 2 |
A few patterns worth noticing when you look at the list. Biology practicals 1 to 5 sit on Paper 1 (Cell biology, Organisation, Infection and response, Bioenergetics), and 6 and 7 sit on Paper 2 (Homeostasis and response; Inheritance, variation and evolution; Ecology). On the Chemistry side, Paper 1 covers practicals 8 to 10 (Atomic structure, Bonding, Quantitative chemistry, Chemical changes, Energy changes), while Paper 2 covers 11 to 13 (Rates and extent, Organic, Chemical analysis, Earth atmosphere, Using resources). Physics Paper 1 covers 14 to 17 (Energy, Electricity, Particle model, Atomic structure), and Paper 2 covers 18 to 21 (Forces, Waves, Magnetism and electromagnetism).
How practical skills are assessed
There isn't a separate practical paper for AQA Combined Science. The practical-skills questions are woven through all six written papers instead.
Across the full qualification, 20% of the marks sit under AO3 (analyse information and ideas; evaluate; develop and improve experimental procedures). On top of that, AQA writes at least 15% of marks across the papers to specifically assess practical skills. In practice that means a handful of questions on every paper that look like method descriptions, variable identification, data analysis or evaluation of an unfamiliar experiment.
AQA keeps coming back to the same four question types for every required practical. The first is describing the method: listing the steps in order, naming the equipment and stating the quantities. Vague answers like "test it with a chemical" score zero. The second is identifying the variables, which means naming the independent variable (what you change), the dependent variable (what you measure), and at least two control variables, and saying how you'd control each one. The third is explaining the results using scientific knowledge: not just "the mass went up", but why. A potato gaining mass in a dilute sugar solution because water moved in by osmosis, from higher water potential to lower, for example. The fourth is evaluating the method: spot a weakness, suggest an improvement.
Six-mark extended-response questions sometimes focus on one required practical end to end. Those need a logical structure: method, science, link to the context of the question.
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This is the one thing people trip over when they revise from a Triple-focused resource. Trilogy drops seven of the practicals that are present on the three separate GCSEs. The maths: Triple is 10 Biology + 8 Chemistry + 10 Physics = 28. Trilogy is 7 + 6 + 8 = 21. The seven that get dropped are the ones that only live in "biology only", "chemistry only" or "physics only" topics on Triple.
| Science | Dropped from Triple → Trilogy | Why it's dropped |
|---|---|---|
| Biology | Microbiology: effect of antiseptics or antibiotics on bacterial growth | Content is biology only within Cell biology |
| Biology | Plant responses: effect of light or gravity on seedling growth | Content is biology only within Homeostasis and response |
| Biology | Decay: effect of temperature on the rate of decay of fresh milk by measuring pH change | Content is biology only within Ecology |
| Chemistry | Titration of a strong acid with a strong alkali | Chemistry-only content within Chemical changes / Quantitative chemistry |
| Chemistry | Chemical tests for ions (flame tests, hydroxide precipitates, carbonate, halide and sulfate tests) | Chemistry-only content within Chemical analysis |
| Physics | Thermal insulators: effect of insulation on energy transfer | Physics-only content within Energy |
| Physics | Light: reflection off a plane mirror and refraction through a rectangular glass block | Physics-only content within Waves |
If you're on Trilogy, don't revise titration, antiseptic plates, phototropism, thermal insulators, decay (pH change in fresh milk), chemical tests for ions, or reflection and refraction as required practicals. They aren't required for you. The underlying science of some of them (waves, for example) does still appear in Trilogy, just not as a required practical you need to describe end to end.
Study tips for the 21 practicals
Reading through the method isn't enough. Required practicals test whether you can think like a scientist, not whether you can recite a recipe. A few things that actually move the needle:
Write the method out from memory for each practical, then check it against your notes. The steps you forgot are the ones that would have cost you marks. Pay attention to the small details AQA likes to ask about: incubating microbiology plates at 25 degrees Celsius rather than 37 (so dangerous pathogens don't thrive), taping agar lids rather than sealing them, inverting plates, controlling the volume of solution in osmosis experiments, blotting potato cylinders before weighing. That level of detail is where the method-description marks sit.
Practise drawing results tables with correct headings, units and number of decimal places. AQA often asks you to complete or draw a table, and the marks are there just for headings-with-units, consistent decimal places and the independent variable in the left column.
Sketch the graphs you'd expect for each practical. For osmosis, a line showing percentage change in mass against concentration that crosses zero at the isotonic point. For photosynthesis, a curve that rises and then plateaus. For force vs extension of a spring, a straight line through the origin until the limit of proportionality. Plotting accurately and labelling axes correctly is worth marks on its own.
Work through past paper questions on each practical. AQA recycles similar wording and structures year on year, and the mark schemes are a goldmine for the exact phrases they want you to use. Our GCSE mock exams guide walks through how to use mock periods to test your recall across all 21 practicals in one go.
When the exam asks you to suggest improvements to a method, think in threes: how to improve accuracy (more precise equipment), how to improve reliability (more repeats and a mean), and how to improve validity (better control of variables).
How Cognito helps
Our AQA GCSE Combined Science courses cover every one of the 21 required practicals through short video lessons, worked examples and exam-style questions. Each practical gets its own lesson walking through the method, the variables, the results and the exam language. You can jump straight into the Combined Science Biology course, the Combined Science Chemistry course or the Combined Science Physics course and track your progress through the whole spec.
For a wider revision framework, our how to revise for GCSE Science guide covers paper structure, timing, and how to layer the required practicals into a week-by-week plan.
